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KMID : 0359319880280020321
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Korean Journal of Veterinary Research
1988 Volume.28 No. 2 p.321 ~ p.325
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BioStudies on enzyme immunoassay for determining progesterone of bovine plasma and its clinical application
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Abstract
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This experiment was carried out to determine the progesterone concentration of bovine plasma by liquid phase double antibody enzyme immunoassay.
The optimum conditions of assay-system, double (first and second) antibody and carrier (normal rabbit serum) were investigated.
The optimum dilution rate of first antibody, second antibody and normal rabbit serum was 10×10³ to 15×10³, 20 and 1×10³ times, respectively.
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